Determination of inhibitors of urokinase-activated fibrinolytic enzyme system by means of gel filtration in human serum.

There are two groups in the inhibitors of fibrinolytic enzyme system : antiplasmins, the direct inhibitors of the enzyme ; and the inhibitors of activators, such as the antistreptokinase, the antiurokinase and the inhibitors of tissue kinases. Moreover, several synthetic compounds such as EACA and AMCHA are also capable of inhibiting fibrinolysis.1) Since the nature of the inhibitors and the mechanism of their action were poorly clarified, our attempts in the isolation, purification, and characterization of the inhibitors in human serum were performed.